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The Science Notes
  • Cell and Molecular Biology
  • Cancer
  • Bacteriology
  • Biotechnology
  • Facts
  • Immunology
  • Genetics
  • Reproductive Biology
  • Vertebrates
  • Invertebrates
  • Virology
  • Anatomy and Physiology
  • Protocols and Techniques
  • Food Science
  • Vitamins
  • Difference between
  • News & Blogs
  • Uncategorized
  • About Us
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Month: April 2020

Posted on April 17, 2020May 24, 2022 Protocols and Techniques

PCR – Polymerase Chain Reaction

There are three major steps in a PCR, which are repeated for 20-35 cycles. 1.  Denaturation at 94°C :During the denaturation, the double strand melts... Read more

Posted on April 17, 2020May 24, 2022 Protocols and Techniques

Plasmid Isolation- Traditional Plasmid Mini Prep Protocol

•Small scale – minipreps – 1-5 ml culture –Yield – 5 to 100 mg plasmid, suitable for restriction analysis, sequencing, PCR  •Medium Scale – Midipreps... Read more

Posted on April 17, 2020May 24, 2022 Protocols and Techniques

Freezing cells

a. Harvest cells as usual (and wash once with complete medium).  b. Resuspend cells in complete medium and determine cell count/viability. Keep cells on ice. ... Read more

Posted on April 17, 2020May 24, 2022 Uncategorized

Centrioles and its Roles

Centriole is a barrel shaped structure found in the area of centrosomes. Centrioles are physically made up of microtubules. The centrioles are in average 200nm... Read more

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