
PCR – Polymerase Chain Reaction
There are three major steps in a PCR, which are repeated for 20-35 cycles. 1. Denaturation at 94°C :During the denaturation, the double strand melts... Read more
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There are three major steps in a PCR, which are repeated for 20-35 cycles. 1. Denaturation at 94°C :During the denaturation, the double strand melts... Read more
•Small scale – minipreps – 1-5 ml culture –Yield – 5 to 100 mg plasmid, suitable for restriction analysis, sequencing, PCR •Medium Scale – Midipreps... Read more
a. Harvest cells as usual (and wash once with complete medium). b. Resuspend cells in complete medium and determine cell count/viability. Keep cells on ice. ... Read more
Centriole is a barrel shaped structure found in the area of centrosomes. Centrioles are physically made up of microtubules. The centrioles are in average 200nm... Read more