Next Generation Sequencing (NGS) Technology : Advancements, Platforms, and Applications
Discover the latest advancements, platforms, and applications of Next Generation Sequencing technology in this comprehensive guide. Read more
Category: Protocols and Techniques
Flow Cytometry : Introduction, Principle, Applications, Limitations
What is Flow cytometry? Flow cytometry is the measuring (metry) of cells (cyto) as they flow (cells in motion) past a detecting device. The technique... Read more
PLANT TISSUE CULTURE
Plant cells have potential to grow as a plant. This potential of the plant is called totipotency. Any component of the plant can grow into... Read more
Pulsed field Gel Electrophoresis (PFGE)
What is Pulsed field Gel Electrophoresis (PFGE)? Pulsed – field gel electrophoresis is a separation technique of large DNA molecules (entire genomic DNA) to produce... Read more
Types of Restriction endonuclease enzymes
Definition Restriction endonuclease enzyme is also known as restriction enzyme or restrictase or molecular scissors which is a type of protein produced by bacteria that... Read more
Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS – PAGE):
What is SDS – PAGE? SDS – PAGE or sodium dodecyl sulfate-polyacrylamide gel electrophoresis is a technique most commonly used in genetic, biotechnology, biochemistry, and... Read more
Bacterial Transformation
Bacterial transformation is the process of transfer of foreign genetic materials from the external environment through the cell membrane. It’s a horizontal form of gene... Read more
Lentivirus Production and Concentration
The production and use of lentivirus should be done in appropriate biological safety cabinet. And lentivirus should be disposed after treatment in 10% bleach. Day... Read more
ELISA TECHNIQUE
Introduction Enzyme-linked immunosorbent assay (ELISA) technique is one of the most familiar names for the production of in vitro diagnostic products, use in medical laboratories,... Read more
Real-time PCR (RT – PCR)
The real-time polymerase chain reaction is also known as quantitative PCR or qPCR or quantitative reverse – transcriptase PCR which is used in the measurement... Read more