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Category: Protocols and Techniques

Next Generation Sequencing
Posted on March 24, 2023March 24, 2023 Cell and Molecular Biology Protocols and Techniques

Next Generation Sequencing (NGS) Technology : Advancements, Platforms, and Applications

Discover the latest advancements, platforms, and applications of Next Generation Sequencing technology in this comprehensive guide. Read more

FLOW CYTOMETRY NOTES
Posted on February 12, 2023February 13, 2023 Cell and Molecular Biology Protocols and Techniques

Flow Cytometry : Introduction, Principle, Applications, Limitations

What is Flow cytometry? Flow cytometry is the measuring (metry) of cells (cyto) as they flow (cells in motion) past a detecting device. The technique... Read more

Posted on February 2, 2023February 12, 2023 Protocols and Techniques

PLANT TISSUE CULTURE

Plant cells have potential to grow as a plant. This potential of the plant is called totipotency. Any component of the plant can grow into... Read more

Posted on January 28, 2022March 19, 2023 Bacteriology Microbiology Protocols and Techniques

Pulsed field Gel Electrophoresis (PFGE)

What is Pulsed field Gel Electrophoresis (PFGE)? Pulsed – field gel electrophoresis is a separation technique of large DNA molecules (entire genomic DNA) to produce... Read more

Posted on June 11, 2021May 24, 2022 Biotechnology Protocols and Techniques

Types of Restriction endonuclease enzymes

Definition Restriction endonuclease enzyme is also known as restriction enzyme or restrictase or molecular scissors which is a type of protein produced by bacteria that... Read more

Posted on February 23, 2021May 24, 2022 Protocols and Techniques

Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS – PAGE):

What is SDS – PAGE? SDS – PAGE or sodium dodecyl sulfate-polyacrylamide gel electrophoresis is a technique most commonly used in genetic, biotechnology, biochemistry, and... Read more

Posted on February 23, 2021May 27, 2022 Bacteriology Cell and Molecular Biology Protocols and Techniques

Bacterial Transformation

Bacterial transformation is the process of transfer of foreign genetic materials from the external environment through the cell membrane. It’s a horizontal form of gene... Read more

Posted on January 20, 2021May 24, 2022 Cell and Molecular Biology Protocols and Techniques

Lentivirus Production and Concentration

The production and use of lentivirus should be done in appropriate biological safety cabinet. And lentivirus should be disposed after treatment in 10% bleach. Day... Read more

Posted on November 24, 2020September 2, 2022 Biotechnology Immunology Microbiology Protocols and Techniques

ELISA TECHNIQUE

Introduction Enzyme-linked immunosorbent assay (ELISA) technique is one of the most familiar names for the production of in vitro diagnostic products, use in medical laboratories,... Read more

Posted on November 8, 2020 Biotechnology Protocols and Techniques

Real-time PCR (RT – PCR)

The real-time polymerase chain reaction is also known as quantitative PCR or qPCR or quantitative reverse – transcriptase PCR which is used in the measurement... Read more

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