Biochemical tests for identification of Gram-positive bacteria
Gram-positive bacteria retain the crystal violet dye by its thick layer of peptidoglycan and appear purple color when observed under the microscope after performing Gram... Read more
Category: Bacteriology
Biochemical tests for identification of Gram-negative bacteria
Gram-negative bacteria are the pink or red-colored bacteria when observed under a microscope after Gram’s differential staining technique. They do not retain the crystal violet... Read more
Staining: Gram staining, Acid Fast Bacilli Staining, Spore Staining, and Capsule Staining
“Stain” is a chemical reagent or dye that is responsible for the discoloration of the specimen. This use of stain for adding contrast for better... Read more
Rapid diagnostic test (RDTs): Uses, Principle, Types, Procedures, Reliability, and Interpretation of test results
Rapid diagnostic tests are serological tests that are used in preliminary medical screening or incase of emergency diagnosis. They either detect antibody (Ab) or antigen... Read more
Biochemical Tests for the Identification of Bacteria: Types, Principle, Procedure, and Examples
Microorganisms undergo the metabolism process to maintain nutritional and functional cellular activities either catabolically or anabolically. Both processes occur simultaneously with the biochemical reaction which... Read more
MICROBIAL CONTROL AND THEIR TYPES
What is Microbial Control? The control (which means either to inhibit or remove and kill) of microorganisms so as to prevent the transmission of diseases... Read more
Wound Infection
Infection of soft tissues associated with pus formation Pus consists PMNs, bacteria, fibrin and other body fluids May be endogenous or exogenous Endogenous: source of... Read more
Antimicrobial Resistance and its Mechanism
Bacterial resistance to drugs is a condition in which the bacteria that were earlier susceptible to antibiotics develop resistance against the same antibiotics and are... Read more
Dilution Tests for Antibiotic Sensitivity Testing (Broth dilution, Agar dilution and E-Test)
Performed to determine the minimum inhibitory concentration (MIC) of an antimicrobial agent. MIC is defined as the lowest concentration of an antimicrobial agent that inhibits... Read more
Tuberculosis : Specimen Collection for Laboratory Diagnosis
Specimen Collection Pulmonary tuberculosis: threat to both patients and others so sample should be collected and handled in a safe manner. Successful identification of pathogens... Read more