• Open the cell culture hood, turn on the light and the blower. Make sure to clean the hood and wipe it with 70% ethanol before use..
  • Vacuum out the media from the cell culture plate.
  • Add PBS (1-2 ml) to wash the plate. Add PBS from the wall of the plate and mix it by rotating the plate.
  • Pipette out the PBS using pipette aid
  • Add Trypsin (1ml). It can be added right on the top of the cell. Spread well.
  • Incubate in CO2 incubator for 10 minutes. Time may vary depending on weather the cells are not adherent anymore or not.
  • 1-2 ml of culture medium can be added and mixed well by pipetting to make sure no clumps are present.
  • Discard the medium, keeping around 0.3 – 0.5 ml of the medium in the plate.
  • Add the medium to make 8-10 ml to dilute and rotate it to spread the cells well.
  • Incubate in CO2 incubator.

Binod G C

I'm Binod G C (MSc), a PhD candidate in cell and molecular biology who works as a biology educator and enjoys scientific blogging. My proclivity for blogging is intended to make notes and study materials more accessible to students.

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