Southern Blotting : Principle, Procedure and Applications
based on the principle of transfer of separated DNA fragments to a carrier membrane (usually nitrocellulose) using gel electrophoresis and subsequent identification of specific DNA fragmentsby labelled probe hybridization. Hybridization is a technique in which a double stranded DNA molecule is formed in between a single stranded DNA probe and a target single stranded DNA. The probes are labeled with a marker and complementary to the target DNA as a result we can detect one molecule of target in a mixture of millions after hybridization as the reactions are specific. Read more