PCR – Polymerase Chain Reaction
There are three major steps in a PCR, which are repeated for 20-35 cycles. 1. Denaturation at 94°C :During the denaturation, the double strand melts... Read more
Author: Binod G C, Ph.D.
Plasmid Isolation- Traditional Plasmid Mini Prep Protocol
•Small scale – minipreps – 1-5 ml culture –Yield – 5 to 100 mg plasmid, suitable for restriction analysis, sequencing, PCR •Medium Scale – Midipreps... Read more
Freezing cells
a. Harvest cells as usual (and wash once with complete medium). b. Resuspend cells in complete medium and determine cell count/viability. Keep cells on ice. ... Read more
Centrioles and its Roles
Centriole is a barrel shaped structure found in the area of centrosomes. Centrioles are physically made up of microtubules. The centrioles are in average 200nm... Read more
Luciferase Reporter Assay – Protocol
LUCIFERASE ASSAY Harvesting the cell (adherent cells) Remove the culture medium 1ml of PBS is added to each well. Scrape out the cells and transfer... Read more
TRANSFORMATION protocol of DH5α Competent E. coli cells
1-5 μl to the cells (1 to 10 ng DNA) is added to the cell and mixed (flicking the tube /not with pipette) Incubate the... Read more
SPLITTING CELLS
Open the cell culture hood, turn on the light and the blower. Make sure to clean the hood and wipe it with 70% ethanol before... Read more
trp Operon
Example of Gene Regulation at steps after Transcription initiation. Where translation is coupled with transcription to achieve regulation of an operon through secondary RNA structure.... Read more
Phylum Echinodermata : General Characteristics
The term Echinodermata (Gr., echinos= spiny, derma=skin), was coined by Jacob Klein Echinoderms are triploblastic, radially symmetrical (adult) often pentamerous, larva with bilateral symmetry, sessile... Read more
Phylum Mollusca : General Characteristics
Phylum Mollusca : General Characteristics (L., mollis=soft) Second largest phylum in number ; first being arthropoda Malacology : Study of Mollusca Conchology : Study of... Read more