Luciferase Reporter Assay – Protocol
LUCIFERASE ASSAY Harvesting the cell (adherent cells) Remove the culture medium 1ml of PBS is added to each well. Scrape out the cells and transfer... Read more
Category: Protocols and Techniques
TRANSFORMATION protocol of DH5α Competent E. coli cells
1-5 μl to the cells (1 to 10 ng DNA) is added to the cell and mixed (flicking the tube /not with pipette) Incubate the... Read more
SPLITTING CELLS
Open the cell culture hood, turn on the light and the blower. Make sure to clean the hood and wipe it with 70% ethanol before... Read more