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Chromatography is generally carried out by organic chemist and biochemists for analysis, isolation and purification. Working principle of Chromatography Types of Chromatography Commonly used chromatographic... Read more
Tips for Efficient Primer Designing Read more
There are three major steps in a PCR, which are repeated for 20-35 cycles. 1. Denaturation at 94°C :During the denaturation, the double strand melts... Read more
•Small scale – minipreps – 1-5 ml culture –Yield – 5 to 100 mg plasmid, suitable for restriction analysis, sequencing, PCR •Medium Scale – Midipreps... Read more
a. Harvest cells as usual (and wash once with complete medium). b. Resuspend cells in complete medium and determine cell count/viability. Keep cells on ice. ... Read more
LUCIFERASE ASSAY Harvesting the cell (adherent cells) Remove the culture medium 1ml of PBS is added to each well. Scrape out the cells and transfer... Read more
1-5 μl to the cells (1 to 10 ng DNA) is added to the cell and mixed (flicking the tube /not with pipette) Incubate the... Read more
Open the cell culture hood, turn on the light and the blower. Make sure to clean the hood and wipe it with 70% ethanol before... Read more