Brewing : Beer making Process
Learn the step-by-step process of beer making, from brewing and fermentation to bottling and aging. Perfect for beer enthusiasts Read more
Category: Protocols and Techniques
MALDI TOF Mass Spectrometry: Principle, Methodology and Applications
Discover the principle, methodology, and applications of MALDI-TOF Mass Spectrometry in this comprehensive guide. Explore now! Read more
Western Blotting : Principle, Procedure and Applications
Learn the principles, procedures, and applications of Western Blotting in this comprehensive guide. Master the technique. Read more
Southern Blotting: Principle, Procedure and Applications
Southern blotting is a molecular biology technique used for DNA detection, characterization, and quantification. Learn more. Read more
DNA MICROARRAY: PRINCIPLE, TYPES, PROCEDURE AND APPLICATIONS
Explore the world of DNA microarrays: Discover principles, types, procedures, and diverse applications for cutting-edge genetic analysis. Read more
Chromatography: Definition, Principle, Types and Application
Chromatography is generally carried out by organic chemist and biochemists for analysis, isolation and purification. Working principle of Chromatography Types of Chromatography Commonly used chromatographic... Read more
TIPS FOR EFFICIENT PRIMER DESIGN
Tips for Efficient Primer Designing Read more
PCR – Polymerase Chain Reaction
There are three major steps in a PCR, which are repeated for 20-35 cycles. 1. Denaturation at 94°C :During the denaturation, the double strand melts... Read more
Plasmid Isolation- Traditional Plasmid Mini Prep Protocol
•Small scale – minipreps – 1-5 ml culture –Yield – 5 to 100 mg plasmid, suitable for restriction analysis, sequencing, PCR •Medium Scale – Midipreps... Read more
Freezing cells
a. Harvest cells as usual (and wash once with complete medium). b. Resuspend cells in complete medium and determine cell count/viability. Keep cells on ice. ... Read more