1-5 μl to the cells (1 to 10 ng DNA) is added to the cell and mixed (flicking the tube /not with pipette)
Incubate the mixture in ice for 30 minutes
Heat shock for 20 seconds in 42°C water bath
Place tubes in ice immediately for 2 minutes.
Add 400 µl of LB media / SOC in the tube.
Incubate the tubes at 37°C shaker for 1 hour at 225 rpm.
After incubation spread 100 µl on the LB (Ampicillin) plates or other selective agar plates.
Incubate plates at 37°C overnight in incubator.