Plasmid Isolation- Traditional Plasmid Mini Prep Protocol
•Small scale – minipreps – 1-5 ml culture –Yield – 5 to 100 mg plasmid, suitable for restriction analysis, sequencing, PCR •Medium Scale – Midipreps... Read more
Category: Protocols and Techniques
Freezing cells
a. Harvest cells as usual (and wash once with complete medium). b. Resuspend cells in complete medium and determine cell count/viability. Keep cells on ice. ... Read more
Luciferase Reporter Assay – Protocol
LUCIFERASE ASSAY Harvesting the cell (adherent cells) Remove the culture medium 1ml of PBS is added to each well. Scrape out the cells and transfer... Read more
TRANSFORMATION protocol of DH5α Competent E. coli cells
1-5 μl to the cells (1 to 10 ng DNA) is added to the cell and mixed (flicking the tube /not with pipette) Incubate the... Read more
SPLITTING CELLS
Open the cell culture hood, turn on the light and the blower. Make sure to clean the hood and wipe it with 70% ethanol before... Read more