About Dr. Binod G C

Flowchart of iPSC lentiviral preparation, transduction, GFP monitoring, puromycin selection, clonal expansion, and validation workflow.

June 10, 2026 Cell and Molecular Biology

iPSC Lentiviral Transduction Protocol with GFP/Puromycin Selection

Detailed research-use protocol for human iPSC lentiviral transduction, including GFP monitoring, puromycin selection, clonal expansion, spin infection considerations, and QC. Read more

The diagram shows three sequential test tubes illustrating the qualitative analysis of proteins containing aromatic amino acids. Step 1 shows a test tube labeled "NITRATION" where concentrated nitric acid ($HNO_3$) is added to a protein sample, beginning to form a light yellow color representing nitrated aromatic amino acids. Step 2 shows the test tube being gently heated over a small burner flame, producing a distinct yellow substance labeled "Xanthoproteic Acid" to ensure reaction completion. Step 3 shows sodium hydroxide ($NaOH$) alkali being added via a dropper, resulting in a dark yellow-orange positive result due to the intensification into an orange salt.

June 3, 2026 Biochemistry Protocols and Techniques

Xanthoproteic Test: Qualitative Protein Analysis Using Nitric Acid

Learn how the xanthoproteic test uses nitric acid to detect aromatic amino acids in proteins, including principle, procedure, results, uses, and limitations. Read more

Scientist in graduation gown reflects on the PhD journey, with laboratory and research imagery symbolizing sacrifice and endurance.

May 25, 2026May 25, 2026 News & Blogs

A PhD Is Not Nothing: An Open Letter to Those Who Dismiss the Journey

PhD is not nothing. This open letter tells the truth about the sacrifice, endurance, failure, and resilience behind earning a PhD in cell and molecular biology—and why the journey deserves respect. Read more

Detailed biomedical illustration of human iPSC-derived liver organoids containing Kupffer cells, hepatic endoderm, endothelial cells, and mesenchymal cells for liver inflammation modeling.

May 1, 2026 Cell and Molecular Biology

Human iPSC-Derived Liver Organoids with Kupffer Cells: Protocol Overview, Research Value, and Applications

Human iPSC-derived liver organoids containing Kupffer cells provide a more advanced in vitro model for studying liver inflammation, immune-cell integration, hepatic dysfunction, and tissue repair. Read more

Illustration of CRISPR-Cas9 gene knockout in a human iPSC-derived macrophage, showing guide RNA targeting DNA inside the immune cell.

May 1, 2026 Cell and Molecular Biology

CRISPR Knockout in iPSC-Derived Macrophages: Optimized Workflow, Benefits, and Research Applications

Human iPSC-derived macrophages offer a scalable model for studying innate immunity, inflammation, and disease biology. This article explains how optimized CRISPR-Cas9 knockout workflows improve functional genomics research in these specialized immune cells. Read more

A comprehensive scientific diagram illustrating the eukaryotic cell cycle and a direct comparison between mitosis and meiosis. The left panel shows a continuous loop of interphase (G1, S, G2 phases) leading to the M phase (mitosis). The right panel provides a side-by-side analysis of mitosis versus meiosis, detailing parent cell (2n) division, chromosome count changes, occurrence in somatic or germ cells, and function

April 10, 2026April 10, 2026 Cell and Molecular Biology

Cell Division: Foundations, Mechanisms, and Modern Research

Explore the essential process of cell division. Learn about the history of cell theory, the mechanics of mitosis and meiosis, and how modern tools like flow cytometry are advancing cancer research. Read more

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