A short Review on E-Cadherin: Structure, Function, Physiological and Pathological importance
Author : Binod G C E-cadherin is one of the members of... Read more
p53 Structure and Function
p53 Structure •p53 is mainly a sequence-specific transcription regulatory protein that binds... Read more
ION CHANNEL SIGNALLING : A SHORT REVIEW
Ion channels are protein molecules that span the lipid bilayer of the... Read more
PCR – Polymerase Chain Reaction
There are three major steps in a PCR, which are repeated for... Read more
Plasmid Isolation- Traditional Plasmid Mini Prep Protocol
•Small scale – minipreps – 1-5 ml culture –Yield – 5 to... Read more
Freezing cells
a. Harvest cells as usual (and wash once with complete medium). b.... Read more
Centrioles and its Roles
Centriole is a barrel shaped structure found in the area of centrosomes.... Read more
Luciferase Reporter Assay – Protocol
LUCIFERASE ASSAY Harvesting the cell (adherent cells) Remove the culture medium 1ml... Read more
TRANSFORMATION protocol of DH5α Competent E. coli cells
1-5 μl to the cells (1 to 10 ng DNA) is added... Read more
SPLITTING CELLS
Open the cell culture hood, turn on the light and the blower.... Read more