PCR – Polymerase Chain Reaction
There are three major steps in a PCR, which are repeated for... Read more
Plasmid Isolation- Traditional Plasmid Mini Prep Protocol
•Small scale – minipreps – 1-5 ml culture –Yield – 5 to... Read more
Freezing cells
a. Harvest cells as usual (and wash once with complete medium). b.... Read more
Centrioles and its Roles
Centriole is a barrel shaped structure found in the area of centrosomes.... Read more
Luciferase Reporter Assay – Protocol
LUCIFERASE ASSAY Harvesting the cell (adherent cells) Remove the culture medium 1ml... Read more
TRANSFORMATION protocol of DH5α Competent E. coli cells
1-5 μl to the cells (1 to 10 ng DNA) is added... Read more
SPLITTING CELLS
Open the cell culture hood, turn on the light and the blower.... Read more
trp Operon
Example of Gene Regulation at steps after Transcription initiation. Where translation is... Read more
Phylum Echinodermata : General Characteristics
The term Echinodermata (Gr., echinos= spiny, derma=skin), was coined by Jacob Klein... Read more
Phylum Mollusca : General Characteristics
Phylum Mollusca : General Characteristics (L., mollis=soft) Second largest phylum in number... Read more